15 Type of Summus periculum Humanum Papillomavirus E6/E7 Gene mRNA
Product nomen
HWTS-CC005A-15 Types of High-Periculum Humanum Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiologia
Cancer cervicus est una e communissimis generibus cancere muliebris in orbe terrarum, et eius eventus prope papillomaviros humanos (HPV), sed parva proportio infectionum HPV in cancrum evolvere potest.Summus periculum HPV inficit cellulas epitheliales cervicales et duos oncoproteinos producit, E6 et E7.Hic interdum potest varias servo cellulosos afficere (qualis est tumor suppressoris servo pRB et p53), cyclum cellularum prolongare, synthesim DNA et stabilitatem genome afficere, et intercedere responsionibus immunibus antiviralibus et antitumoribus.
Channel
| Channel | Component | Genotype temptavit |
| FAM | HPV Reactio Buffer 1 | HPV16、31、33、35、51、52、58 |
| VIC/HEX | Humanum β-actin gene | |
| FAM | HPV Reactio Buffer 2 | HPV 18、39、45、53、56、59、66、68 |
| VIC/HEX | Human INS gene |
Technical Parameters
| Repono | Liquid: ≤-18℃ |
| Fasciae vita | IX mensibus |
| Specimen Type | ceruicis exfoliated cellula |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Copia/mL |
| Applicabilis Instrumenta | Acta Biosystems (VII)D Real-time PCR System Acta Biosystems (VII)D Fast Verus-Tempus PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-time PCR Systems LightCycler®480 Real-time PCR systema LineGene 9600 Plus Real-time PCR Detection System MA-6000 Real-time Quantitatis Thermal Cycler BioRad CFX96 Real-time PCR System BioRad CFX Opus 96 Real-time PCR System |
Opus O
Extraction reagens commendatur: Macro & Micro-test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) ab Jiangsu Macro & Micro-test Med-Tech Co. .Voluminis elutionis commendatum 50μL est.Si specimen non perfecte concoctum est, redde illud ad redigendum 4 gradum.Et tunc probate secundum mandatum utendi.
Commendatur extraction reagens: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Extractio facienda est secundum instructiones ad usum stricte (In gradu V, duplex intentio solutionis DNaseI laborantis, hoc est, accipe 20μL ex RNase-Free DNaseI (1500U) solutionem stirpis in novam RNase-Free centrifugium tubum; adde 60μL ex RDD quiddam et leniter misce).Voluminis evolutionis commendata 60μL est.Si specimen non perfecte concoctum est, redde illud ad 5 gradus redigendis.Et tunc probate secundum mandatum utendi.
