Human BRAF Gene V600E Mutation
Product nomen
HWTS-TM007-Human BRAF Gene V600E Mutatio Detection Kit (Fluorescens PCR)
Testimonium
CE
Epidemiologia
Plusquam 30 genera mutationum BRAF inventae sunt, quarum circiter 90% in exon 15, ubi mutatio V600E frequentissima censetur, id est thymine(T) in positione 1799 in exon 15 mutatur. adenine (A), inde in substitutione valinae (V) positionis 600 per acidum glutamicum (E) in interdum producto.BRAF mutationes in tumoribus malignis communiter inveniuntur, sicut melanoma, cancer colorectalis, cancer thyreoidea, et cancer pulmonis.BRAF gene mutatio intelligendi facta est necessitas ad protegendum EGFR-TKis et BRAF gene mutationis-iaculatorum medicamentorum clinicorum iaculis medicamentorum aegrorum qui prodesse possunt.
Channel
| FAM | V600E mutatio, imperium internum |
Technical Parameters
| Repono | ≤-18℃ |
| Fasciae vita | IX mensibus |
| Specimen Type | paraffin-embedded pathologicae TEXTUS exemplaria |
| CV | -5.0% |
| Ct | ≤38 |
| LoD | Utere kits ad deprehendere LoD quale imperium respondentem.a) sub 3ng/μL background generis silvestris, 1% mutatio rate deprehendi potest in quiddam constanter reactione;b) sub 1% mutatione rate, mutatione 1×103Exempla / mL in bestiis-genus background de 1×105Codices / mL in quiddam reactionis deprehensum stabiliter possunt;c) Reactio IC Buffer potest deprehendere infimas detectionis limites qualitatis SW3 ditionis societatis internae. |
| Instrumenta applicabilia: | Acta Biosystems (VII)D Real-time PCR SystemsActa Biosystems (VII)CCC Real-Tempus PCR Systems, QuantStudio® 5 Real-Time PCR Systems LightCycler®480 Real-time PCR systema BioRad CFX96 Real-time PCR System |
Opus O
Extraction reagentia commendatur: QIAGEN scriptor QIAamp DNA FFPE Tissue Ornamentum (56404), Paraffin-embeded Tissue DNA Celeri Extraction Ornamentum (DP330) fabricatum a Tiangen Biotech (Beijing) Co., Ltd.
